Mamori Anezaki

Mamori is intelligent, responsible and intensely protective of Sena. Having defended him from bullies since childhood, she has become accustomed to intervening whenever she believes someone is exploiting him. Hiruma's threats and abrasive behavior therefore immediately put the two at odds.

She also has a strong nurturing streak. Mamori cooks, looks after injured or exhausted players and frequently acts as the practical adult in a club filled with eccentric personalities. Her kindness does not make her passive; she openly challenges Hiruma when she believes he has gone too far.

Mamori is academically gifted but unusually poor with computers. She compensates by handling enormous amounts of tactical information on paper, turning her preference for analogue work into part of her effectiveness as a manager.

Mamori has an exceptional memory and aptitude for learning. Soon after joining the club, she studies the rules of American football intensively enough to become a reliable authority on them despite beginning with little knowledge of the sport.

Her greatest contribution is tactical analysis. She records formations and tendencies, studies opposing teams and helps interpret what is happening during games. Mamori and Hiruma develop a system of hand signals that lets her communicate observations to him without opponents understanding their exchange.

She has little athletic importance compared with Deimon's players, but her organizational ability is essential to a club whose small roster forces it to extract maximum value from every available player.

Mamori begins as Sena's protector rather than a football enthusiast. Because she believes he is merely the club's secretary, she initially does not realize that the mysterious Eyeshield 21 she watches on the field is Sena himself.

Her investment in the team steadily becomes independent of protecting him. She learns the sport, works alongside Hiruma on strategy and becomes a genuine football manager rather than an outsider supervising Sena.

Mamori eventually discovers that Sena is Eyeshield 21, forcing her to accept that the boy she has always protected has chosen to face dangerous opponents for himself. His growing independence changes their relationship without weakening their childhood bond.

Sena Kobayakawa is Mamori's childhood friend. She has protected Sena Kobayakawa since elementary school and initially struggles to recognize that he must learn to confront difficulties without her intervention.

Youichi Hiruma is Mamori's principal strategic partner and frequent antagonist in everyday club life. She objects to Youichi Hiruma's intimidation and manipulation, while he nevertheless comes to rely heavily on her intelligence and sideline analysis.

Ryokan Kurita welcomes Mamori into the club and provides a gentler contrast to its intimidating quarterback.

Suzuna Taki becomes another important female presence around the Devil Bats and frequently works alongside Mamori in supporting the team.